Pyrophosphate as a central energy carrier in the hydrogen-producing extremely thermophilic Caldicellulosiruptor saccharolyticus

被引：32

作者：

Bielen, Abraham A. M. ^{[1
]}

Willquist, Karin ^{[2
]}

Engman, Jakob ^{[2
]}

van der Oost, John ^{[1
]}

van Niel, Ed W. J. ^{[2
]}

Kengen, Serve W. M. ^{[1
]}

机构：

[1] Wageningen UR, Microbiol Lab, Wageningen, Netherlands

[2] Lund Univ, Lund, Sweden

来源：

FEMS MICROBIOLOGY LETTERS | 2010年 / 307卷 / 01期

关键词：

Caldicellulosiruptor saccharolyticus; pyrophosphate; H plus -PPase; phosphofructokinase; glycolysis; ESCHERICHIA-COLI; DEPENDENT PHOSPHOFRUCTOKINASE; INORGANIC PYROPHOSPHATE; CLOSTRIDIUM-ACETOBUTYLICUM; PHOSPHATE DIKINASE; METABOLISM; ATP; PYRUVATE; ENZYMES; EXPRESSION;

D O I：

10.1111/j.1574-6968.2010.01957.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The role of inorganic pyrophosphate (PPi) as an energy carrier in the central metabolism of the extremely thermophilic bacterium Caldicellulosiruptor saccharolyticus was investigated. In agreement with its annotated genome sequence, cell extracts were shown to exhibit PPi-dependent phosphofructokinase and pyruvate phosphate dikinase activity. In addition, membrane-bound pyrophosphatase activity was demonstrated, while no significant cytosolic pyrophosphatase activity was detected. During the exponential growth phase, high PPi levels (approximately 4 +/- 2 mM) and relatively low ATP levels (0.43 +/- 0.07 mM) were found, and the PPi/ATP ratio decreased 13-fold when the cells entered the stationary phase. Pyruvate kinase activity appeared to be allosterically affected by PPi. Altogether, these findings suggest an important role for PPi in the central energy metabolism of C. saccharolyticus.

引用

页码：48 / 54

页数：7

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