Off-target effects associated with long dsRNAs in Drosophila RNAi screens

被引：41

作者：

Moffat, Jason

Reiling, Jan H.

Sabatini, David M.

机构：

[1] Whitehead Inst, Cambridge, MA 02142 USA

[2] MIT, Dept Biol, Cambridge, MA 02139 USA

来源：

TRENDS IN PHARMACOLOGICAL SCIENCES | 2007年 / 28卷 / 04期

关键词：

D O I：

10.1016/j.tips.2007.02.009

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Evidence of off-target effects (OTEs) associated with small interfering (si)RNAs (19-29 bp) in mammalian cells has existed for several years. Two recent articles demonstrate that short sequences within long double-stranded (ds)RNAs frequently cause undesirable OTEs in cultured Drosophila cells. These results reveal the potential for high false-positive rates in RNA interference (RNAi) screens using long dsRNAs and highlight the need for screening with multiple, non-overlapping long dsRNAs or siRNAs. Discovering multiple potent siRNAs with minimal off-target profiles for each target transcript will be invaluable for genome-based studies of gene function and for personalized RNAi therapeutics.

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页码：149 / 151

页数：3

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