Human 5′→3′ exonuclease Xrn2 promotes transcription termination at co-transcriptional cleavage sites

被引：321

作者：

West, S ^{[1
]}

Gromak, N ^{[1
]}

Proudfoot, NJ ^{[1
]}

机构：

[1] Univ Oxford, Sir William Dunn Sch Pathol, Oxford OX1 3RE, England

来源：

NATURE | 2004年 / 432卷 / 7016期

关键词：

D O I：

10.1038/nature03035

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Eukaryotic protein-encoding genes possess poly(A) signals that define the end of the messenger RNA and mediate downstream transcriptional termination by RNA polymerase II (Pol II)(1). Termination could occur through an 'anti-termination' mechanism whereby elongation factors dissociate when the poly(A) signal is encountered, producing termination-competent Pol II2,3. An alternative 'torpedo' model postulated that poly(A) site cleavage provides an unprotected RNA 5' end that is degraded by 5' --> 3' exonuclease activities ( torpedoes) and so

引用

页码：522 / 525

页数：4

共 25 条

[21] Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences [J].

Strausberg, RL ;

Feingold, EA ;

Grouse, LH ;

Derge, JG ;

Klausner, RD ;

Collins, FS ;

Wagner, L ;

Shenmen, CM ;

Schuler, GD ;

Altschul, SF ;

Zeeberg, B ;

Buetow, KH ;

Schaefer, CF ;

Bhat, NK ;

Hopkins, RF ;

Jordan, H ;

Moore, T ;

Max, SI ;

Wang, J ;

Hsieh, F ;

Diatchenko, L ;

Marusina, K ;

Farmer, AA ;

Rubin, GM ;

Hong, L ;

Stapleton, M ;

Soares, MB ;

Bonaldo, MF ;

Casavant, TL ;

Scheetz, TE ;

Brownstein, MJ ;

Usdin, TB ;

Toshiyuki, S ;

Carninci, P ;

Prange, C ;

Raha, SS ;

Loquellano, NA ;

Peters, GJ ;

Abramson, RD ;

Mullahy, SJ ;

Bosak, SA ;

McEwan, PJ ;

McKernan, KJ ;

Malek, JA ;

Gunaratne, PH ;

Richards, S ;

Worley, KC ;

Hale, S ;

Garcia, AM ;

Gay, LJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (26) :16899-16903

[22] Processing of 3′-extended read-through transcripts by the exosome can generate functional mRNAs [J].

Torchet, C ;

Bousquet-Antonelli, C ;

Milligan, L ;

Thompson, E ;

Kufel, J ;

Tollervey, D .

MOLECULAR CELL, 2002, 9 (06) :1285-1296

[23] RNAi-mediated PTB depletion leads to enhanced exon definition [J].

Wagner, EJ ;

Garcia-Blanco, MA .

MOLECULAR CELL, 2002, 10 (04) :943-949

[24] Autoregulation of polypyrimidine tract binding protein by alternative splicing leading to nonsense-mediated decay [J].

Wollerton, MC ;

Gooding, C ;

Wagner, EJ ;

Garcia-Blanco, MA ;

Smith, CWJ .

MOLECULAR CELL, 2004, 13 (01) :91-100

[25] Cloning and mapping of the XRN2 gene to human chromosome 20p11.1-p11.2 [J].

Zhang, M ;

Yu, L ;

Xin, YR ;

Hu, PR ;

Fu, Q ;

Yu, C ;

Zhao, SY .

GENOMICS, 1999, 59 (02) :252-254

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