Human 5′→3′ exonuclease Xrn2 promotes transcription termination at co-transcriptional cleavage sites

被引：321

作者：

West, S ^{[1
]}

Gromak, N ^{[1
]}

Proudfoot, NJ ^{[1
]}

机构：

[1] Univ Oxford, Sir William Dunn Sch Pathol, Oxford OX1 3RE, England

来源：

NATURE | 2004年 / 432卷 / 7016期

关键词：

D O I：

10.1038/nature03035

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Eukaryotic protein-encoding genes possess poly(A) signals that define the end of the messenger RNA and mediate downstream transcriptional termination by RNA polymerase II (Pol II)(1). Termination could occur through an 'anti-termination' mechanism whereby elongation factors dissociate when the poly(A) signal is encountered, producing termination-competent Pol II2,3. An alternative 'torpedo' model postulated that poly(A) site cleavage provides an unprotected RNA 5' end that is degraded by 5' --> 3' exonuclease activities ( torpedoes) and so

引用

页码：522 / 525

页数：4

共 25 条

[11] Cotranscriptionally formed DNA:RNA hybrids mediate transcription elongation impairment and transcription-associated recombination [J].

Huertas, P ;

Aguilera, A .

MOLECULAR CELL, 2003, 12 (03) :711-721

[12] Regulation of alternative splicing by a transcriptional enhancer through RNA pol II elongation [J].

Kadener, S ;

Fededa, JP ;

Rosbash, M ;

Kornblihtt, AR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (12) :8185-8190

[13] Nonsense-mediated mRNA decay in mammalian cells involves decapping, deadenylating, and exonucleolytic activities [J].

Lejeune, F ;

Li, XJ ;

Maquat, LE .

MOLECULAR CELL, 2003, 12 (03) :675-687

[14] A POLY(A) ADDITION SITE AND A DOWNSTREAM TERMINATION REGION ARE REQUIRED FOR EFFICIENT CESSATION OF TRANSCRIPTION BY RNA POLYMERASE-II IN THE MOUSE BETA-MAJ-GLOBIN GENE [J].

LOGAN, J ;

FALCKPEDERSEN, E ;

DARNELL, JE ;

SHENK, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (23) :8306-8310

[15] EM visualization of transcription by RNA polymerase II: Downstream termination requires a poly(A) signal but not transcript cleavage [J].

Osheim, YN ;

Proudfoot, NJ ;

Beyer, AL .

MOLECULAR CELL, 1999, 3 (03) :379-387

[16] Processing of the precursors to small nucleolar RNAs and rRNAs requires common components [J].

Petfalski, E ;

Dandekar, T ;

Henry, Y ;

Tollervey, D .

MOLECULAR AND CELLULAR BIOLOGY, 1998, 18 (03) :1181-1189

[17] New perspectives on connecting messenger RNA 3' end formation to transcription [J].

Proudfoot, N .

CURRENT OPINION IN CELL BIOLOGY, 2004, 16 (03) :272-278

[18] HOW RNA POLYMERASE-II TERMINATES TRANSCRIPTION IN HIGHER EUKARYOTES [J].

PROUDFOOT, NJ .

TRENDS IN BIOCHEMICAL SCIENCES, 1989, 14 (03) :105-110

[19] A small nucleolar RNA:ribozyme hybrid cleaves a nucleolar RNA target in vivo with near-perfect efficiency [J].

Samarsky, DA ;

Ferbeyre, G ;

Bertrand, E ;

Singer, RH ;

Cedergren, R ;

Fournier, MJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (12) :6609-6614

[20] 5'-EXONUCLEASE-2 OF SACCHAROMYCES-CEREVISIAE - PURIFICATION AND FEATURES OF RIBONUCLEASE ACTIVITY WITH COMPARISON TO 5'-EXONUCLEASE-1 [J].

STEVENS, A ;

POOLE, TL .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (27) :16063-16069

← 1 2 3 →