Copper-dependent toxicity in SH-SY5Y neuroblastoma cells involves mitochondrial damage

被引:70
作者
Arciello, M [1 ]
Rotilio, G [1 ]
Rossi, L [1 ]
机构
[1] Univ Roma Tor Vergata, Dept Biol, I-00133 Rome, Italy
关键词
copper; SH-SY5Y; neurodegeneration; mitochondria; Wilson's disease;
D O I
10.1016/j.bbrc.2004.12.022
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Treatment of SH-SY5Y human neuroblastoma cells with copper sulphate (50-300 muM) in complete medium for 24 h caused an increase in the level of the metal both in whole cells and in isolated mitoplasts. Toxic effects of copper resulted in the impairment of the capability of mitochondrial dehydrogenases to reduce a tetrazolium salt, and, to a lesser extent, in the loss of the integrity of the plasma membrane. The mechanism of toxicity involved the production of reactive oxygen species, amplified by the presence of ascorbate. Decreases in the levels of several mitochondrial proteins (subunits of complex I, complex V, and of the pyruvate dehydrogenase complex) were observed. These findings demonstrate that mitochondria are an early and susceptible target of copper-mediated oxidative stress in neuronal cells and support the hypothesis that rnitochondrial damage triggers the neurodegenerative processes associated with copper overload in Wilson's disease. (C) 2004 Elsevier Inc. All rights reserved.
引用
收藏
页码:454 / 459
页数:6
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