CeO2 nanoparticles induce DNA damage towards human dermal fibroblasts in vitro

被引：158

作者：

Auffan, Melanie ^{[1
]}

Rose, Jerome ^{[1
]}

Orsiere, Thierry ^{[2
]}

De Meo, Michel ^{[2
]}

Thill, Antoine ^{[3
]}

Zeyons, Ophelie ^{[3
]}

Proux, Olivier ^{[4
]}

Masion, Armand ^{[1
]}

Chaurand, Perrine ^{[1
]}

Spalla, Olivier ^{[3
]}

Botta, Alain ^{[2
]}

Wiesner, Mark R. ^{[5
]}

Bottero, Jean-Yves ^{[1
]}

机构：

[1] Aix Marseille Univ, CNRS, CEREGE, UMR6635, F-13545 Aix En Provence, France

[2] Univ Mediterranee, Fac Med & Pharm, Lab Biogenotoxicol & Mutagenese Environm EA 1784, Marseille, France

[3] CEA Saclay, Lab Interdisciplinaire Org Nanometr & Supramol, F-91191 Gif Sur Yvette, France

[4] Univ Grenoble 1, CNRS, UMR, LGIT, F-38402 St Martin Dheres, France

[5] Duke Univ, Durham, NC USA

来源：

NANOTOXICOLOGY | 2009年 / 3卷 / 02期

基金：

美国国家科学基金会;

关键词：

Cerium oxide nanoparticle; genotoxicity; cell; oxidative stress; redox; CERIUM OXIDE NANOPARTICLES; OXIDATIVE STRESS; NITRIC-OXIDE; CYTOTOXICITY; PROTECTION; TOXICITY; CANCER;

D O I：

10.1080/17435390902788086

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Cerium dioxide nanoparticles have been proposed for an increasing number of applications in biomedicine, cosmetic, as polishing materials and also as byproducts from automotive fuel additives. The aim of this study was to examine the potential in vitro cyto- and genotoxicity of nano-sized CeO2 (7 nm) on human dermal fibroblasts. By combining a physicochemical and a (geno)toxicological approach, we defined the causal mechanisms linking the physico-chemical properties of nano-CeO2 with their biological effects. Using X-ray absorption spectroscopy, we observed a reduction of 21 +/- 4% of the Ce4+ atoms localized at the surface of CeO2 nanoparticles due to the interactions with organic molecules present in biological media. These particles induced strong DNA lesions and chromosome damage related to an oxidative stress. These genotoxic effects occurred at very low doses, which highlighted the importance of a genotoxicological approach during the assessment of the toxicity of nanoparticles.

引用

页码：161 / U115

页数：12

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