This work identifies an ISCR1 -related bla(CTX-M-14) gene, which has never been reported before, from a clinical isolate of Escherichia coli. The bla(CTX-M-14) gene was preceded by an ISCR1 element that was followed by a class I integron containing three different insert gene cassettes, i.e., dfrA12, orfF, and aad42.