Precise analysis of primary amino acids in urine by an automated high-performance liquid chromatography method: comparison with ion-exchange chromatography

被引：29

作者：

Fekkes, D ^{[1
]}

Voskuilen-Kooyman, A

Jankie, R

Huijmans, J

机构：

[1] Dept Psychiat, Sect Pathophysiol Behav, Rotterdam, Netherlands

[2] Erasmus Univ, Dept Clin Genet, NL-3000 DR Rotterdam, Netherlands

来源：

JOURNAL OF CHROMATOGRAPHY B | 2000年 / 744卷 / 01期

关键词：

amino acids;

D O I：

10.1016/S0378-4347(00)00234-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A precise, simple and rapid method for the quantitative determination of primary amino acids in urine based on high-performance liquid chromatography and o-phthaldialdehyde pre-column derivatization is described. All primary urinary amino acids could be determined within 49 min (injection to injection). Amino acid concentrations in 40 urinary samples were measured by this method and the results were compared with those measured by ion-exchange chromatography. The correlation coefficient for the common amino acids was greater than 0.90. This is the first study in which such a detailed comparison has been made on urine samples. It appeared that the method described is an excellent alternative to the classical ion-exchange method for the quantitation of urinary amino acids. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：183 / 188

页数：6

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