Amperometric biosensor based on direct electrochemistry of hemoglobin in poly-allylamine (PAA) film

被引：18

作者：

Kafi, A. K. M.

Lee, Dong-Yun

Park, Sang-Hyun

Kwon, Young-Soo

机构：

[1] Dong A Univ, Dept Elect Engn, Pusan 604714, South Korea

[2] Dong A Univ, CIIPMS, Pusan 604714, South Korea

来源：

THIN SOLID FILMS | 2007年 / 515卷 / 12期

关键词：

hemoglobin; poly-allylamine; electrochemistry; biosensor;

D O I：

10.1016/j.tsf.2006.10.028

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Hemoglobin (Hb) was immobilized in poly-allylamine (PAA) film onto the gold electrode by layer by layer (LBL) method. The modified electrode exhibited a pair of well-defined peaks during cyclic voltammetry, which was attributed from the direct electron transfer of heme proteins. The immobilized Hb showed an excellent electrocatalytical response to the reduction of hydrogen peroxide. The sensor exhibited a fast response and high sensitivity. Through the use of optimized conditions, the linear range for H2O2 detection was from 2.5 x 10(-6) M to 5 x 10(-4) M with detection limit of 0.2 mu M. The proposed biosensor showed long-lasting stability and excellent reproducibility. (c) 2006 Elsevier B.V. All rights reserved.

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收藏

页码：5179 / 5183

页数：5

相关论文

共 23 条

[1] Ordered electrochemically active films of hemoglobin, didodecyldimethylammonium ions, and clay [J].

Chen, XL ;

Hu, NF ;

Zeng, YH ;

Rusling, JF ;

Yang, J .

LANGMUIR, 1999, 15 (20) :7022-7030

[2] Electron-transfer reactivity and enzymatic activity of hemoglobin in a SP sephadex membrane [J].

Fan, CH ;

Wang, HY ;

Sun, S ;

Zhu, DX ;

Wagner, G ;

Li, GX .

ANALYTICAL CHEMISTRY, 2001, 73 (13) :2850-2854

[3] Direct electron transfer and characterization of hemoglobin immobilized on a Au colloid-cysteamine-modified gold electrode [J].

Gu, HY ;

Yu, AM ;

Chen, HY .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 2001, 516 (1-2) :119-126

[4] Direct electrochemistry of hemoglobin in egg-phosphatidylcholine films and its catalysis to H2O2 [J].

Han, XJ ;

Huang, WM ;

Jia, JB ;

Dong, SJ ;

Wang, EK .

BIOSENSORS & BIOELECTRONICS, 2002, 17 (09) :741-746

[5] DNA-Cu(II) complex as a novel electrocatalyst for a hydrogen peroxide sensor [J].

Hasebe, Y ;

Gu, TT .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 2005, 576 (01) :177-181

[6]

Hiroyuki O., 1998, ELECTROCHIM ACTA, V43, P1581

[7] Hydrogen peroxide biosensor based on DNA-Hb modified gold electrode [J].

Kafi, AKM ;

Yin, F ;

Shin, HK ;

Kwon, YS .

THIN SOLID FILMS, 2006, 499 (1-2) :420-424

[8] Prussian-Blue-based amperometric biosensors in flow-injection analysis [J].

Karyakin, AA ;

Karyakina, EE ;

Gorton, L .

TALANTA, 1996, 43 (09) :1597-1606

[9] GENERAL EXPRESSION OF THE LINEAR POTENTIAL SWEEP VOLTAMMOGRAM IN THE CASE OF DIFFUSIONLESS ELECTROCHEMICAL SYSTEMS [J].

LAVIRON, E .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1979, 101 (01) :19-28

[10] A reagentless amperometric biosensor based on the coimmobilization of horseradish peroxidase and methylene green in a modified zeolite matrix [J].

Liu, BH ;

Yan, F ;

Kong, JL ;

Deng, JQ .

ANALYTICA CHIMICA ACTA, 1999, 386 (1-2) :31-39