When metagenomics meets stable-isotope probing: progress and perspectives

被引：124

作者：

Chen, Yin ^{[1
]}

Murrell, J. Colin ^{[1
]}

机构：

[1] Univ Warwick, Dept Biol Sci, Coventry CV4 7AL, W Midlands, England

来源：

TRENDS IN MICROBIOLOGY | 2010年 / 18卷 / 04期

基金：

英国自然环境研究理事会;

关键词：

MULTIPLE DISPLACEMENT AMPLIFICATION; COMMUNITY STRUCTURE; RIBOSOMAL-RNA; MICROBIAL-POPULATIONS; DNA LIBRARIES; IDENTIFICATION; LINKING; SOIL; ENRICHMENT; BACTERIUM;

D O I：

10.1016/j.tim.2010.02.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The application of metagenomics, the culture-independent capture and subsequent analysis of genomic DNA from the environment, has greatly expanded our knowledge of the diversity of microbes and microbial protein families; however, the metabolic functions of many microorganisms remain largely unknown. DNA stable-isotope probing (DNA-SIP) is a recently developed method in which the incorporation of stable isotope from a labelled substrate is used to identify the function of microorganisms in the environment. The technique has now been used in conjunction with metagenomics to establish links between microbial identity and particular metabolic functions. The combination of DNA-SIP and metagenomics not only permits the detection of rare low-abundance species from metagenomic libraries but also facilitates the detection of novel enzymes and bioactive compounds.

引用

页码：157 / 163

页数：7

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