A new tool for superoxide and nitric oxide radicals determination using suitable enzymatic sensors

被引:37
作者
Campanella, L [1 ]
Persi, L [1 ]
Tomassetti, M [1 ]
机构
[1] Univ Rome La Sapienza, Dept Chem, I-00185 Rome, Italy
关键词
superoxide; nitric oxide; radicals; detection; biosensors;
D O I
10.1016/S0925-4005(00)00415-9
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A new tool for radical determination is investigated. Superoxide and nitric oxide radicals have been determined using their modulating action on three different enzymatic sensors based on tyrosinase, superoxide dismutase (SOD), and galactose oxidase (GAO), respectively. Basic research was carried nut to study the inhibition or activation effect of the two radicals considered on the enzyme sensor's response and experimentally evidencing the correlation between the modulating action of the biosensor response and the radical concentration in solution. Results are shown as linear ranges, which differ according to the biosensor used and the radical determined. (C) 2000 Elsevier Science S.A. All rights reserved.
引用
收藏
页码:351 / 359
页数:9
相关论文
共 23 条
  • [1] ADEYOYU D, 1995, ANAL CHIM ACTA, V305, P57
  • [2] BARNUM TE, 1969, ENZYME HDB, P115
  • [3] A modified amperometric electrode for the determination of free radicals
    Campanella, L
    Favero, G
    Tomassetti, M
    [J]. SENSORS AND ACTUATORS B-CHEMICAL, 1997, 44 (1-3) : 559 - 565
  • [4] THE EFFECT OF ORGANIC-SOLVENT PROPERTIES ON THE RESPONSE OF A TYROSINASE ENZYME SENSOR
    CAMPANELLA, L
    FAVERO, G
    SAMMARTINO, MP
    TOMASSETTI, M
    [J]. TALANTA, 1994, 41 (06) : 1015 - 1023
  • [5] DETERMINATION OF PHENOL IN WASTES AND WATER USING AN ENZYME SENSOR
    CAMPANELLA, L
    BEONE, T
    SAMMARTINO, MP
    TOMASSETTI, M
    [J]. ANALYST, 1993, 118 (08) : 979 - 986
  • [6] A new organic phase enzyme electrode for the analysis of organophosphorus pesticides and carbamates
    Campanella, L
    De Luca, S
    Sammartino, MP
    Tomassetti, M
    [J]. ANALYTICA CHIMICA ACTA, 1999, 385 (1-3) : 59 - 71
  • [7] Selective membrane sensors for free radical analysis based on potentiometric and CHEMFET devices
    Campanella, L
    Favero, G
    Occhionero, E
    Tomassetti, M
    [J]. ANALUSIS, 1998, 26 (05) : 223 - 228
  • [8] Superoxide dismutase biosensors for superoxide radical analysis
    Campanella, L
    Favero, G
    Tomassetti, M
    [J]. ANALYTICAL LETTERS, 1999, 32 (13) : 2559 - 2581
  • [9] CAMPANELLA L, 1999, SENS MICR P 3 IT C G, P219
  • [10] CAMPANELLA L, 1998, ANN CHIM, V88, P687