Chromatographic properties of monolithic silica capillary columns for polar and nonpolar compounds in reversed-phase HPLC

被引：15

作者：

Kobayashi, H ^{[1
]}

Kajiwara, W ^{[1
]}

Inui, Y ^{[1
]}

Hara, T ^{[1
]}

Hosoya, K ^{[1
]}

Ikegami, T ^{[1
]}

Tanaka, N ^{[1
]}

机构：

[1] Kyoto Inst Technol, Dept Polymer Sci & Engn, Sakyo Ky, Kyoto 6068585, Japan

来源：

CHROMATOGRAPHIA | 2004年 / 60卷 / Suppl 1期

关键词：

column liquid chromatography; capillary liquid chromatography; reversed-phase; monolithic silica;

D O I：

10.1365/s10337-004-0326-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Retention properties of monolithic silica C-18 columns were examined for polar and nonpolar compounds. Monolithic silica columns were prepared from two kinds of starting materials, tetromethoxysilane (abbreviated as TMOS) or a mixture of TMOS and methyltrimethoxysilane (abbreviated as Hybrid), and modified with dimethyloctadecylchlorosilane (C-18). Retention factors of various compounds on Hybrid-C-18 are about twice as much as that on TMOS-C-18. The two types of columns showed very similar retention selectivity with few exceptions. TMOS-C-18 showed slight preference toward planar polynuclear aromatic hydrocarbons (PAHs) compared to bulky aromatic hydrocarbons, while Hybrid-C-18 showed opposite tendency. Some TMOS-C-18 columns showed peak tailing for PAHs, and also for basic compounds in an acidic mobile phase, while Hybrid-C-18 and majority of TMOS-C-18 columns resulted in good peak shape for these compounds.

引用

页码：S19 / S25

页数：7

共 17 条

[1]

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