Optimization of extraction of Tremella fuciformis polysaccharides and its antioxidant and antitumour activities in vitro

被引：171

作者：

Bin, Chen ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Peoples Hosp 9, Sch Med, Dept Gen Surg, Shanghai 200011, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2010年 / 81卷 / 02期

关键词：

Tremella fuciformis polysaccharides; Response surface methodology; Extraction; Antioxidant; Antitumour; RESPONSE-SURFACE METHODOLOGY; MUSHROOMS; LEAF;

D O I：

10.1016/j.carbpol.2010.02.039

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

Optimization of extraction of Tremella fuciformis polysaccharides was investigated using response surface methodology in this paper. T. fuciformis polysaccharides were extracted using boiling water at different extraction temperatures (80-100 degrees C), times (3.5-4.5 h) and ratios of solvent to raw material (4-6). The effect of extraction conditions on the yield of T. fuciformis polysaccharides were studied using a three-level three-factor Box-Behnken design. The results showed that the highest yield (3.08%) of T. fuciformis polysaccharides was reached at extraction temperature 100 degrees C, extraction time 4.5 h and ratio of solvent to raw material 5 (v/v). In addition, T. fuciformis polysaccharides could scavenge superoxide anion and hydroxyl radicals. At last, it could still be found that antitumour activities of T. fuciformis polysaccharides increased from 73.4% to 92.1% with increasing concentration of polysaccharides. Pharmacology experiment indicated that T. fuciformis polysaccharides was useful to the therapy of free radical injury and cancer diseases. (C) 2010 Published by Elsevier Ltd.

引用

页码：420 / 424

页数：5

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