Identification and discrimination of Burkholderia pseudomallei, B-mallei, and B-thailandensis by real-time PCR targeting type III secretion system genes

被引：81

作者：

Thibault, FM ^{[1
]}

Valade, E ^{[1
]}

Vidal, DR ^{[1
]}

机构：

[1] Ctr Rech, Serv Sante Armees, F-38702 La Tronche, France

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2004年 / 42卷 / 12期

关键词：

D O I：

10.1128/JCM.42.12.5871-5874.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Burkholderia pseudomallei and B. mallei are two highly pathogenic bacteria, responsible for melioidosis and glanders, respectively. The two are closely related and can also be mistaken for B. thailandensis, a nonpathogenic species. To improve their differential identification, we describe a hydrolysis probe-based real-time PCR method using the uneven distribution of type III secretion system genes among these three species.

引用

页码：5871 / 5874

页数：4

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