Reconstitution of DNA segregation driven by assembly of a prokaryotic actin homolog

被引：165

作者：

Garner, Ethan C.

Campbell, Christopher S.

Weibel, Douglas B.

Mullins, R. Dyche ^{[1
]}

机构：

[1] Univ Calif San Francisco, UCB Nanomed Dev Ctr, San Francisco, CA 94158 USA

[2] Marine Biol Lab, Physiol Course, Woods Hole, MA 02543 USA

[3] Univ Wisconsin, Dept Biochem, Madison, WI 53706 USA

来源：

SCIENCE | 2007年 / 315卷 / 5816期

关键词：

D O I：

10.1126/science.1138527

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Multiple unrelated polymer systems have evolved to partition DNA molecules between daughter cells at division. To better understand polymer-driven DNA segregation, we reconstituted the three-component segregation system of the R1 plasmid from purified components. We found that the ParR/parC complex can construct a simple bipolar spindle by binding the ends of ParM filaments, inhibiting dynamic instability, and acting as a ratchet permitting incorporation of new monomers and riding on the elongating filament ends. Under steady-state conditions, the dynamic instability of unattached ParM filaments provides the energy required to drive DNA segregation.

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页码：1270 / 1274

页数：5

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