Zirconia based nucleic acid sensor for Mycobacterium tuberculosis detection

被引：58

作者：

Das, Maumita ^{[1
]}

Sumana, Gajjala ^{[1
]}

Nagarajan, R. ^{[2
]}

Malhotra, B. D. ^{[1
,3
]}

机构：

[1] Natl Phys Lab, Ctr Biomol Elect, Dept Sci & Technol, New Delhi 110012, India

[2] Univ Delhi, Dept Chem, Delhi 110007, India

[3] Chungnam Natl Univ, Ctr NanoBioEngn & Spintron, Taejon 305764, South Korea

来源：

APPLIED PHYSICS LETTERS | 2010年 / 96卷 / 13期

关键词：

biomedical equipment; biosensors; diseases; DNA; electrochemical sensors; electrochemistry; Fourier transform spectra; gold; infrared spectra; molecular biophysics; nanobiotechnology; nanostructured materials; particle size; patient diagnosis; scanning electron microscopy; thin films; voltammetry (chemical analysis); X-ray diffraction; zirconium compounds; HYDROGEN-PEROXIDE BIOSENSOR; FILM; IMMOBILIZATION; NANOPARTICLES; DEPOSITION;

D O I：

10.1063/1.3293447

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

Nanostructured zirconium oxide (ZrO2) film (particle size similar to 35 nm), electrochemically deposited onto gold(Au) surface, has been used to immobilize 21-mer oligonucleotide probe (ssDNA) specific to Mycobacterium tuberculosis by utilizing affinity between oxygen atom of phosphoric group and zirconium to fabricate DNA biosensor. This DNA-ZrO2/Au bioelectrode, characterized using x-ray diffraction, Fourier transform infrared spectroscopy, cyclic voltammetry, and scanning electron microscopy techniques, can be used for early and rapid diagnosis of M. tuberculosis with detection limit of 0.065 ng/mu L within 60s.

引用

页数：3

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