Scanning probe-based frequency-dependent microrheology of polymer gels and biological cells

被引：374

作者：

Mahaffy, RE ^{[1
]}

Shih, CK

MacKintosh, FC

Käs, J

机构：

[1] Univ Texas, Dept Phys, Austin, TX 78712 USA

[2] Univ Michigan, Dept Phys, Ann Arbor, MI 48109 USA

[3] Univ Michigan, Div Biophys Res, Ann Arbor, MI 48109 USA

[4] Univ Texas, Inst Cell & Mol Biol, Austin, TX 78712 USA

[5] Univ Texas, Texas Mat Inst, Austin, TX 78712 USA

来源：

PHYSICAL REVIEW LETTERS | 2000年 / 85卷 / 04期

关键词：

D O I：

10.1103/PhysRevLett.85.880

中图分类号：

O4 [物理学];

学科分类号：

0702 ;

摘要：

A new scanning probe-based microrheology approach is used to quantify the frequency-dependent viscoelastic behavior of both fibroblast cells and polymer gels, The scanning probe shape was modified using polystyrene beads for a defined surface area nondestructively deforming the sample. An extended Hertz model is introduced to measure the frequency-dependent storage and loss moduli even for thin cell samples. Control measurements of the polyacrylamide gels compare well with conventional rheological data. The cells show a viscoelastic signature similar to in vitro actin gels.

引用

页码：880 / 883

页数：4

共 18 条

[1]

BOHM J, IN PRESS BIOPHYS J

[2] MEASURING THE NANOMECHANICAL PROPERTIES AND SURFACE FORCES OF MATERIALS USING AN ATOMIC FORCE MICROSCOPE [J].