Microfabrication and test of a three-dimensional polymer hydro-focusing unit for flow cytometry applications

被引:80
作者
Yang, R
Feeback, DL
Wang, WJ
机构
[1] Louisiana State Univ, Dept Mech Engn, Baton Rouge, LA 70803 USA
[2] NASA Johnson Space Ctr, Houston, TX 77058 USA
关键词
SU-8; three-dimensional hydro-focusing; microfluidic; microchannel; cytometer;
D O I
10.1016/j.sna.2004.09.001
中图分类号
TM [电工技术]; TN [电子技术、通信技术];
学科分类号
0808 ; 0809 ;
摘要
This paper details a novel true three-dimensional (3D) hydro-focusing microcell sorter for micro-flow cytometry applications. The unit was microfabricated by means of SU-83D lithography. The 3D microstructure for coaxial sheathing was designed, microfabricated, and tested. Three-dimensional hydro-focusing capability was demonstrated with an experiment to sort labeled tanned sheep erythrocytes (red blood cells). This polymer hydro-focusing microstructure is easily microfabraicted and integrated with other polymer microfluidic structures. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:259 / 267
页数:9
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