High-throughput RNAi screening to dissect cellular pathways: A how-to guide

被引：31

作者：

Falschlehner, Christina

Steinbrink, Sandra

Erdmann, Gerrit

Boutros, Michael ^{[1
]}

机构：

[1] German Canc Res Ctr, D-69120 Heidelberg, Germany

来源：

BIOTECHNOLOGY JOURNAL | 2010年 / 5卷 / 04期

关键词：

High throughput; Methods; RNA interference; Screening; Cellular signaling; FUNCTIONAL GENOMIC ANALYSIS; DOUBLE-STRANDED-RNA; C-ELEGANS; MAMMALIAN-CELLS; INTERFERING RNA; SIRNA; DELIVERY; GENES; IDENTIFICATION; REGULATORS;

D O I：

10.1002/biot.200900277

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

RNA interference (RNAi) has become a powerful tool to dissect cellular pathways and characterize gene functions. The availability of genome-wide RNAi libraries for various model organisms and mammalian cells has enabled high-throughput RNAi screenings. These RNAi screens successfully identified key components that had previously been missed in classical forward genetic screening approaches and allowed the assessment of combined loss-of-function phenotypes. Crucially, the quality of RNAi screening results depends on quantitative assays and the choice of the right biological context. In this review, we provide an overview on the design and application of high-throughput RNAi screens as well as data analysis and candidate validation strategies.

引用

收藏

页码：368 / 376

页数：9

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