Role of calcineurin and Mpk1 in regulating the onset of mitosis in budding yeast

被引：117

作者：

Mizunuma, M ^{[1
]}

Hirata, D ^{[1
]}

Miyahara, K ^{[1
]}

Tsuchiya, E ^{[1
]}

Miyakawa, T ^{[1
]}

机构：

[1] Hiroshima Univ, Grad Sch Engn, Dept Mol Biotechnol, Higashihiroshima 739, Japan

来源：

NATURE | 1998年 / 392卷 / 6673期

关键词：

D O I：

10.1038/32695

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Signalling via calcium is probably involved in regulating eukaryotic cell proliferation, but details of its mechanism of action are unknown(1,2). In Schizosaccharomyces pombe, the onset of mitosis is determined by activation of a complex of the p36(cdc2) protein kinase and a cyclin protein that is specific to the G2 phase of the cell cycle. This activation requires dephosphorylation of p34(cdc2) (ref. 3). Wee1, a tyrosine kinase that inhibits p34(cdc2) by phosphorylating it, is needed to determine the length of G2 phase, Here we show that calcium-activated pathways in Saccharomyces cerevisiae control the onset of mitosis by regulating Swe1, a Wee1 homologue. Zds1 (also known as Oss1 and Hst1) (refs 4-7) is important in repressing the transcription of SWE1 in G2 phase. In the presence of high calcium levels, cells lacking Zds1 are delayed in entering mitosis. Calcineurin(8-11) and Mpk1 (refs 12, 13) regulate Swe1 activation at the transcriptional and posttranslational levels, respectively, and both are required for the calcium-induced delay in G2 phase. These cellular pathways also induce a G2-phase delay In response to hypotonic shock.

引用

页码：303 / 306

页数：4

共 25 条

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