Involvement of the yeast metacaspase Yca1 in ubp10Δ-programmed cell death

被引：51

作者：

Bettiga, M ^{[1
]}

Calzari, L ^{[1
]}

Orlandi, I ^{[1
]}

Alberghina, L ^{[1
]}

Vai, M ^{[1
]}

机构：

[1] Univ Milan, Dipartimento Biotecnol & Biosci, I-20126 Milan, Italy

来源：

FEMS YEAST RESEARCH | 2004年 / 5卷 / 02期

关键词：

apoptosis; deubiquitinating enzyme; metacaspase; ascorbic acid;

D O I：

10.1016/j.femsyr.2004.07.005

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

UBP10 encodes a deubiquitinating enzyme of Saccharomyces cerevisiae. Its inactivation results in a complex phenotype characterized by a subpopulation of cells that exhibits the typical cellular markers of apoptosis. Here, we show that additional deletion of YCA1, coding for the yeast metacaspase, suppressed the ubp10 disruptant phenotype. Moreover, YCA1 overexpression, without any external stimulus, had a detrimental effect on growth and viability of ubp10 cells accompanied by an increase of apoptotic cells. This response was completely abrogated by ascorbic acid addition. We also observed that cells lacking UBP10 had an endogenous caspase activity, revealed by incubation in vivo with FITC-labeled VAD-fmk. All these results argue in favour of an involvement of the yeast metacaspase in the active cell death triggered by loss of UBP10 function. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：141 / 147

页数：7

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