New PCR test that recognizes all human prototypes of enterovirus: Application for clinical diagnosis

被引:8
作者
Bourlet, T
Caro, V
Minjolle, S
Jusselin, I
Pozzetto, B
Crainic, R
Colimon, R
机构
[1] Univ Rennes 1, Lab Bacteriol Virol, Fac Med, F-35043 Rennes, France
[2] Fac Med, Lab Bacteriol Virol, GIMAP, St Etienne, France
[3] Inst Pasteur, Lab Epidemiol Mol Enterovirus, Paris, France
关键词
D O I
10.1128/JCM.41.4.1750-1752.2003
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We describe a new PCR test (Penter RT-PCR) that recognizes all 64 prototypes of enterovirus. Sixty clinical samples were analyzed in parallel with this Penter RT-PCR and previously described PCR tests: 34 and 32 samples tested positive, respectively. This assay is suitable for use in clinical diagnosis, and its ability to amplify all known serotypes makes it more useful than other consensus PCR tests.
引用
收藏
页码:1750 / 1752
页数:3
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