DNA Methylation Increases Nucleosome Compaction and Rigidity

被引：208

作者：

Choy, John S. ^{[3
]}

Wei, Sijie ^{[1
]}

Lee, Ju Yeon ^{[1
]}

Tan, Song ^{[2
]}

Chu, Steven ^{[3
]}

Lee, Tae-Hee ^{[1
]}

机构：

[1] Penn State Univ, Dept Chem, University Pk, PA 16802 USA

[2] Penn State Univ, Dept Biochem & Mol Biol, University Pk, PA 16802 USA

[3] Stanford Univ, Dept Phys, Bio X Program, Stanford, CA 94305 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2010年 / 132卷 / 06期

关键词：

HISTONE DEACETYLASE; 5-METHYLCYTOSINE; CHROMATIN; PATTERNS; MECP2;

D O I：

10.1021/ja910264z

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Cytosine methylation oil CpG dinucleotides is all essential epigenetic modification ill eukaryotes. How DNA methylation modulates nucleosome structure and dynamics has been a long-standing question. We implemented a single-molecule method to monitor the effects of DNA methylation on the structure and dynamics of mononucleosomes. Our studies show that DNA methylation induces a more compact and rigid nucleosome structure, providing a physical basis for how DNA methylation might contribute to regulating chromatin structure.

引用

页码：1782 / +

页数：4

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