Surface modification via plasma polymerization of allylamine for antibody immobilization

被引：24

作者：

Basarir, Fevzihan ^{[1
]}

Cuong, Nguyen ^{[1
]}

Song, Woo-Keun ^{[2
]}

Yoon, Tae-Ho ^{[1
]}

机构：

[1] Gwangju Inst Sci & Technol, Dept Mat Sci & Engn, 1 Oryong Dong, Kwangju 500712, South Korea

[2] Gwangju Inst Sci & Technol, Dept Life Sci, Kwangju 500712, South Korea

来源：

MACROMOLECULAR SYMPOSIA | 2007年 / 249卷

关键词：

allylamine; amine density; biosensor; plasma poymerization;

D O I：

10.1002/masy.200750310

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 [高分子化学与物理]; 080501 [材料物理与化学]; 081704 [应用化学];

摘要：

Continuous wave and pulsed plasma polymerization coatings of allylamine were investigated for antibody immobilization as a function of plasma power, monomer pressure and treatment time, and duty cycle. Conditions were optimized by evaluating the surface amine density of plasma polymer coated samples before and after aging in dry ethanol for 3 hrs. in addition, plasma polymer coatings were characterized by contact angle analyzer, alpha-step, and FT-IR/ATR. The continuous wave plasma polymerization provided amine density of 4.8 molecules/nm(2) at the optimum condition of 20 W, 1 min, and 60 mTorr, while the pulsed plasma polymerization coating resulted in further increased amine density to 5.2 molecules/nm(2) at the duty cycle of 60/100.

引用

页码：61 / 66

页数：6

共 14 条

[1]

Characterization of pulsed plasma polymerization allylamine as an adhesion layer for DNA adsorption/hybridization [J].