Optical neuronal guidance

被引:8
作者
Ehrlicher, Allen [1 ]
Betz, Timo [1 ]
Stuhrmann, Bjoern [1 ]
Goegler, Michael [1 ]
Koch, Daniel [1 ]
Franze, Kristian [1 ]
Lu, Yunbi [1 ]
Kaes, Josef [1 ]
机构
[1] Univ Leipzig, Lehrstuhl Phys Weicher Mat, Fak Phys & Geowissensch, D-04103 Leipzig, Germany
来源
CELL MECHANICS | 2007年 / 83卷
关键词
D O I
10.1016/S0091-679X(07)83021-4
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
We present a novel technique to noninvasively control the growth and turning behavior of an extending neurite. A highly focused infrared laser, positioned at the leading edge of a neurite, has been found to induce extension/turning toward the beam's center. This technique has been used successfully to guide NG108-15 and PC12 cell lines [Ehrlicher, A., Betz, T., Stuhrmann, B., Koch, D. Milner, V. Raizen, M. G., and Kas, J. (2002). Guiding neuronal growth with light. Proc. Natl. Acad. Sci. USA 99, 16024-16028], as well as primary rat and mouse cortical neurons [Stuhrmann, B., Goegler, M., Betz, T., Ehrlicher, A., Koch, D., and Kas, J. (2005). Automated tracking and laser micromanipulation of cells. Rev. Sci. Instr. 76, 035105]. Optical guidance may eventually be used alone or with other methods for controlling neurite extension in both research and clinical applications.
引用
收藏
页码:495 / +
页数:29
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