Easily fabricated magnetic traps for single-cell applications

被引：20

作者：

Koschwanez, John H. ^{[1
]}

Carlson, Robert H. ^{[1
]}

Meldrum, Deirdre R. ^{[1
]}

机构：

[1] Univ Washington, Dept Elect Engn, Seattle, WA 98195 USA

来源：

REVIEW OF SCIENTIFIC INSTRUMENTS | 2007年 / 78卷 / 04期

关键词：

D O I：

10.1063/1.2722400

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

We describe a simple and inexpensive method of fabricating single cell magnetic traps within a polydimethylsiloxane (PDMS) device. These traps were developed as part of an automated system that captures individual yeast cells in a microfluidic device and analyzes each cell as it buds. To make the traps, PdCl2 catalyst is rubbed with vinyl foam onto plasma-patterned PDMS, and then Co-Ni-B alloy is electrolessly deposited onto the catalyst at a moderate temperature. We demonstrate individual yeast cell capture and estimate the capture force (1.9-4.4 pN) by measuring the flow speed required to remove the cell from its trap in a microfluidic channel.

引用

页数：5

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