Antioxidant effect of polyamines on erythrocyte cell membrane lipoperoxidation after free-radical damage

被引:14
作者
Farriol, M [1 ]
Segovia-Silvestre, T [1 ]
Venereo, Y [1 ]
Orta, X [1 ]
机构
[1] Hosp Gen Valle Hebron, Ctr Invest Bioquim & Biol Mol, CIBBIM, E-08035 Barcelona, Spain
关键词
malondialdehyde; TBARs; polyamines; antioxidants; free radicals;
D O I
10.1002/ptr.1073
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
This in vitro study determined the effect of three doses each (100, 500 and 1000 mum) of putrescine, spermidine and spermine on malondialdehyde (MDA) release in red blood cells (R-BCs) from healthy individuals after hydrogen peroxide stimulation (10 mm). Twenty-two volunteers, 9 males and 13 females, aged 41.5 +/- 16.4 years, were studied. MDA was measured by thiobarbituric reaction (TBARs) and the results were calculated using epsilon = 1.56 x 10(5). The analysis of variance (ANOVA) demonstrated a statistically significant overall decrease in MDA release in the polyamine-exposed cells (p < 0.0001) when compared with unexposed cells. Individual analysis of each polyamine separately showed a 52% decrease in MDA release with added spermine and a 39.5% decrease with added spermidine (P < 0.001). No evaluable effect was found for putrescine. There was no correlation between the effect produced and the three doses of spermidine or spermine added, indicating a non dose-dependent action. Copyright (C) 2003 John Wiley Sons, Ltd.
引用
收藏
页码:44 / 47
页数:4
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