Ultrathin films of charged polysaccharides assembled alternately with linear polyions

被引:113
作者
Lvov, Y
Onda, M
Ariga, K
Kunitake, T
机构
[1] Kyushu Univ, Fac Engn, Fukuoka 812, Japan
[2] Kurume Res Ctr, JST, Supermol Project, Kurume, Fukuoka 839, Japan
关键词
alternate layer-by-layer assembly; chitosan; chondroitin sulfate; quartz crystal microbalance; non-contact atomic force microscopy;
D O I
10.1080/09205063.1998.9753060
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
As a means of preparation of biocompatible molecular surfaces, an alternate assembly of charged polysaccharides and oppositely-charged synthetic polymers was conducted. Cationic chitosan was assembled alternately with anionic poly(sodium styrenesulfonate) (PSS) at pH 4. Regular film growth and its dependence on ionic strength were detected by the quartz crystal microbalance (QCM) method. Averaged film thicknesses for the chitosan + PSS layer were 15, 31, 46, and 69 Angstrom, respectively, when 0, 0.25, 0.5, and 1 M of NaCl was contained in aqueous chitosan. Adsorption of chitosan did not reach saturation in 20 min at 0 M NaCl, while the adsorption became saturated within 6 min with 0.25 M NaCl. Anionic sodium chondroitin sulfate was also assembled in alternation with cationic poly(dimethyldiallylammonium, chloride) (PDDA) at pH 6.5. The adsorption of chondroitin sulfate was less sensitive to ionic strength. Surface morphology of chitosan-PSS films was investigated by non-contact atomic force microscopy (AFM) observation. Maximum height difference and Ra value for a 1000 x 1000 nm area were 11 and 0.69 nm, respectively, indicating the formation of a molecularly flat surface by alternate layer-by-layer adsorption.
引用
收藏
页码:345 / 355
页数:11
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