Development of fluorescent film sensors for the detection of divalent copper

被引:338
作者
Zheng, Y
Orbulescu, J
Ji, X
Andreopoulos, FM [1 ]
Pham, SM
Leblanc, RM
机构
[1] Univ Miami, Sch Med, Dept Surg, Miami, FL 33136 USA
[2] Univ Miami, Dept Biomed Engn, Miami, FL 33136 USA
[3] Univ Miami, Dept Chem, Coral Gables, FL 33124 USA
关键词
D O I
10.1021/ja0293610
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Monolayers of several peptide lipids at air-water and air-solid interfaces were prepared using Langmuir and Langmuir-Blodgett (LB) film techniques, and tested as fluorescent sensors for copper ions in aqueous phase. In one method, both the ionophore and the fluorophore were in the same molecule (lipid A), so intramolecular interaction was responsible for the fluorescence quenching of monolayers of this lipid. In the other method, ionophore and fluorophore were located on two different molecules (lipids B and C) so the intramolecular coupling does not exist; instead the fluorescence quenching was realized by a through-space interaction mechanism. Several experimental techniques, including pi-A isotherm, epifluorescence microscopy, and absorption and emission spectroscopies were used to study the different characteristics of copper ion effect on the properties of the lipid monolayers. Additionally, the fluorescence quenching properties of the Langmuir monolayers were found to be transferred to the one-layer LB films. On LB films, the fluorescence response presented a clear selectivity for copper ions in comparison with several other transition metal ions. Further, an excellent reversibility was observed: the fluorescence was switched OFF by immersing the solid substrate in copper ion solution and ON by washing with HCl solution. The intermolecular approach used here seems to be a very flexible and general method to design surfaceoriented fluorescent sensors to meet different analytic purposes.
引用
收藏
页码:2680 / 2686
页数:7
相关论文
共 33 条
[1]   Recent developments in transition metal ion detection by luminescent chemosensors [J].
Bargossi, C ;
Fiorini, MC ;
Montalti, M ;
Prodi, L ;
Zaccheroni, N .
COORDINATION CHEMISTRY REVIEWS, 2000, 208 :17-32
[2]   A new selective fluorescence chemosensor for Cu(II) in water [J].
Beltramello, M ;
Gatos, M ;
Mancin, F ;
Tecilla, P ;
Tonellato, U .
TETRAHEDRON LETTERS, 2001, 42 (52) :9143-9146
[3]   Self-assembling in surfactant aggregates: An alternative way to the realization of fluorescence chemosensors for Cu(II) ions [J].
Berton, M ;
Mancin, F ;
Stocchero, G ;
Tecilla, P ;
Tonellato, U .
LANGMUIR, 2001, 17 (24) :7521-7528
[4]   Synthesis of a novel thiazole based dipeptide chemosensor for Cu(II) in water [J].
Bhattacharya, S ;
Thomas, M .
TETRAHEDRON LETTERS, 2000, 41 (52) :10313-10317
[5]   A NOVEL LYSINE-PROTECTING PROCEDURE FOR CONTINUOUS-FLOW SOLID-PHASE SYNTHESIS OF BRANCHED PEPTIDES [J].
BYCROFT, BW ;
CHAN, WC ;
CHHABRA, SR ;
HONE, ND .
JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1993, (09) :778-779
[6]   Signaling recognition events with fluorescent sensors and switches [J].
de Silva, AP ;
Gunaratne, HQN ;
Gunnlaugsson, T ;
Huxley, AJM ;
McCoy, CP ;
Rademacher, JT ;
Rice, TE .
CHEMICAL REVIEWS, 1997, 97 (05) :1515-1566
[7]   A fluorescent chemosensor for the copper(II) ion [J].
DeSantis, G ;
Fabbrizzi, L ;
Licchelli, M ;
Mangano, C ;
Sacchi, D ;
Sardone, N .
INORGANICA CHIMICA ACTA, 1997, 257 (01) :69-76
[8]  
Desvergne J.P., 1997, NATO ASI SERIES
[9]   Fluorescent sensors for transition metals based on electron-transfer and energy-transfer mechanisms [J].
Fabbrizzi, L ;
Licchelli, M ;
Pallavicini, P ;
Perotti, A ;
Taglietti, A ;
Sacchi, D .
CHEMISTRY-A EUROPEAN JOURNAL, 1996, 2 (01) :75-82
[10]  
Fabbrizzi L., 1999, TRANSITION METALS SU, V5, P93