Aptamer/Graphene Oxide Nanocomplex for in Situ Molecular Probing in Living Cells

被引：937

作者：

Wang, Ying ^{[1
,2
]}

Li, Zhaohui ^{[2
]}

Hu, Dehong ^{[2
]}

Lin, Chiann-Tso ^{[2
]}

Li, Jinghong ^{[1
]}

Lin, Yuehe ^{[2
]}

机构：

[1] Tsinghua Univ, Dept Chem, Key Lab Bioorgan Phosphorus Chem & Chem Biol, Beijing 100084, Peoples R China

[2] Pacific NW Natl Lab, Richland, WA 99352 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2010年 / 132卷 / 27期

基金：

中国国家自然科学基金;

关键词：

QUANTUM DOTS; GRAPHENE; DNA; APTAMERS; DELIVERY;

D O I：

10.1021/ja103169v

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Graphene has shown fascinating applications in bio-nanotechnology, including DNA sensing, protein assays, and drug delivery. However, exploration of graphene with intracellular monitoring and in situ molecular probing is still at an early stage. In this regard, we have designed an aptamer-carboxyfluorescein (FAM)/graphene oxide nanosheet (GO-nS) nanocomplex to investigate its ability for molecular probing in living cells. Results demonstrate that uptake of aptamer-FAM/GO-nS nanocomplex and cellular target monitoring were realized successfully. The dramatic delivery, protection, and sensing capabilities of GO-nS in living cells indicate that graphene oxide could be a robust candidate for many biological fields, such as DNA and protein analysis, gene and drug delivering, and intracellular tracking.

引用

页码：9274 / 9276

页数：3

共 18 条

[11] Rapid Sequencing of Individual DNA Molecules in Graphene Nanogaps [J].

Postma, Henk W. Ch. .

NANO LETTERS, 2010, 10 (02) :420-425

[12]

Rosi NL, 2006, SCIENCE, V312, P1027, DOI 10.1126/science.1125559

[13] Imaging and tracking of tat peptide-conjugated quantum dots in living cells: new insights into nanoparticle uptake, intracellular transport, and vesicle shedding [J].

Ruan, Gang ;

Agrawal, Amit ;

Marcus, Adam I. ;

Nie, Shuming .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (47) :14759-14766

[14] Nano-Graphene Oxide for Cellular Imaging and Drug Delivery [J].

Sun, Xiaoming ;

Liu, Zhuang ;

Welsher, Kevin ;

Robinson, Joshua Tucker ;

Goodwin, Andrew ;

Zaric, Sasa ;

Dai, Hongjie .

NANO RESEARCH, 2008, 1 (03) :203-212

[15] Constraint of DNA on Functionalized Graphene Improves its Biostability and Specificity [J].

Tang, Zhiwen ;

Wu, Hong ;

Cort, John R. ;

Buchko, Garry W. ;

Zhang, Youyu ;

Shao, Yuyan ;

Aksay, Ilhan A. ;

Liu, Jun ;

Lin, Yuehe .

SMALL, 2010, 6 (11) :1205-1209

[16] Molecular Engineering of DNA: Molecular Beacons [J].

Wang, Kemin ;

Tang, Zhiwen ;

Yang, Chaoyong James ;

Kim, Youngmi ;

Fang, Xiaohong ;

Li, Wei ;

Wu, Yanrong ;

Medley, Colin D. ;

Cao, Zehui ;

Li, Jun ;

Colon, Patrick ;

Lin, Hui ;

Tan, Weihong .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2009, 48 (05) :856-870

[17] Nitrogen-Doped Graphene and Its Application in Electrochemical Biosensing [J].

Wang, Ying ;

Shao, Yuyan ;

Matson, Dean W. ;

Li, Jinghong ;

Lin, Yuehe .

ACS NANO, 2010, 4 (04) :1790-1798

[18]

Xia JL, 2009, NAT NANOTECHNOL, V4, P505, DOI [10.1038/NNANO.2009.177, 10.1038/nnano.2009.177]

← 1 2 →