High-throughput crystallography at an affordable cost: The TB Structural Genomics Consortium Crystallization Facility

被引：50

作者：

Rupp, B ^{[1
]}

机构：

[1] Lawrence Livermore Natl Lab, Macromol Crystallog & TB Struct Genom Consortium, Livermore, CA 94551 USA

来源：

ACCOUNTS OF CHEMICAL RESEARCH | 2003年 / 36卷 / 03期

关键词：

D O I：

10.1021/ar020021t

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The crystallization facility of the TB Structural Genomics Consortium, one of nine P50 structural genomics centers sponsored by the National Institutes of Health, provides TB consortium members with automated crystallization, data collection, and basic molecular replacement structure solution up to bias-minimized maps. In contrast to venture capital -funded commercial enterprises, the TB consortium facilities are decentralized and aim to develop high-throughput crystallography methods and automation on a comparatively small budget. In addition to financial constraints, the logistics and organization of a production environment differ considerably from academic settings. The TB Structural Genomics Consortium crystallization facility may thus provide a model for cost-effective, efficient high-throughput crystallography. Processes and methods presented in this review should assist academic institutions planning to invest in high-throughput structural biology to assess both the rewards and risks of establishing structural genomics programs.

引用

页码：173 / 181

页数：9

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