RFLP analysis of mitochondrial DNA in eggplant and related Solanum species

被引：20

作者：

Isshiki, S ^{[1
]}

Suzuki, S ^{[1
]}

Yamashita, K ^{[1
]}

机构：

[1] Saga Univ, Fac Agr, Dept Biotechnol & Plant Breeding, Saga 8408502, Japan

来源：

GENETIC RESOURCES AND CROP EVOLUTION | 2003年 / 50卷 / 02期

关键词：

eggplant; mitochondrial DNA; phylogenetic relationships; RFLP; Solanum;

D O I：

10.1023/A:1022954229295

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

RFLP analysis of mitochondrial DNA ( mtDNA) was performed by the Southern hybridization method using total DNA extracted from eggplant, Solanum melongena L., and six related Solanum species, S. surattense Burm. (i.e. S. virginianum L.), S. torvum Swartz, S. gilo Raddi (i.e. S. aethiopicum), S. integrifolium Poir. (i.e. S. aethiopicum), S. indicum auct. non L. (i.e. S. violaceum Ort.) and S. sanitwongsei Craib. Forty-one fragments were detected by the analysis using 12 combinations of four restriction enzymes and three probes of mtDNA clones from rice. Thirty-four out of the 41 fragments were polymorphic among the species, whereas the other seven were monomorphic. This RFLP analysis of mtDNA is demonstrated to be appropriate for assessing phylogenetic relationships in eggplant and related Solanum species at the interspecific level.

引用

页码：133 / 137

页数：5

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