Polymer-based microfluidic devices for biomedical applications

被引：14

作者：

Hupert, ML ^{[1
]}

Witek, MA ^{[1
]}

Wang, Y ^{[1
]}

Mitchell, MW ^{[1
]}

Liu, Y ^{[1
]}

Bejat, Y ^{[1
]}

Nikitopoulos, DE ^{[1
]}

Goettert, J ^{[1
]}

Murphy, MC ^{[1
]}

Soper, SA ^{[1
]}

机构：

[1] Louisiana State Univ, Dept Chem, Baton Rouge, LA 70803 USA

来源：

MICROFLUIDICS, BIOMEMS, AND MEDICAL MICROSYSTEMS | 2003年 / 4982卷

关键词：

microfluidic device; micromachining techniques; PMMA; PC; HARMs; PCR; microarray;

D O I：

10.1117/12.480771

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Two types of microfluidic bioanalytical systems were designed and fabricated in polymer substrates using the LIGA process. A continuous flow polymerase chain reaction (CFPCR) microfluidic device was fabricated in polycarbonate (PC), which utilized isothermal zone and shuttling the sample through each zone to achieve amplification. A 20-cycle PCR amplification of a fragment of a plasmid DNA template was achieved in 5.3 min. The results were comparable to those obtained in commercial laboratory-scale PCR system. The second system consisted of a microchip contating a low-density array assembled into the microfluidic channel, which was hot-embossed in poly(methyl methacrylate) (PMMA). The detection of low-abundant mutations in gene fragments (K-ras) that carry point mutations with high diagnostic value for colorectal cancer was successfully performed. The array was accessed using microfluidics in order to enhance the kinetics associated with hybridization.

引用

页码：52 / 64

页数：13

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