Dynamics of EF-G interaction with the ribosome explored by classification of a heterogeneous cryo-EM dataset

被引：74

作者：

Gao, HX

Valle, M

Ehrenberg, M

Frank, J

机构：

[1] Hlth Res Inc, Howard Hughes Med Inst, Albany, NY 12201 USA

[2] Biomed Ctr, Dept Cell & Mol Biol, S-75124 Uppsala, Sweden

[3] SUNY Albany, Dept Biomed Sci, Albany, NY 12201 USA

来源：

JOURNAL OF STRUCTURAL BIOLOGY | 2004年 / 147卷 / 03期

关键词：

cryo-electron microscopy; reference-based alignment; supervised classification; translation; translocation;

D O I：

10.1016/j.jsb.2004.02.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method of supervised classification using two available structure templates was applied to investigate the possible heterogeneity existing in a large cryo-EM dataset of an Escherichia coli 70S ribosome-EF-G complex. Two subpopulations showing the ribosome in distinct conformational states, related by a ratchet-like rotation of the 30S subunit with respect to the 50S subunit, were extracted from the original dataset. The possible presence of additional intermediate states is discussed. (C) 2004 Elsevier Inc. All rights reserved.

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收藏

页码：283 / 290

页数：8

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