Hydrazone Ligation Strategy to Assemble Multifunctional Viral Nanoparticles for Cell Imaging and Tumor Targeting

被引：121

作者：

Brunel, Florence M. ^{[1
,2
]}

Lewis, John D. ^{[1
]}

Destito, Giuseppe ^{[1
,3
,4
]}

Steinmetz, Nicole F. ^{[1
,3
]}

Manchester, Marianne ^{[1
,3
]}

Stuhlmann, Heidi ^{[1
]}

Dawson, Philip E. ^{[1
,2
]}

机构：

[1] Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA

[2] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA

[3] Scripps Res Inst, Ctr Integrat Mol Biosci, La Jolla, CA 92037 USA

[4] Univ Magna Graecia di Catanzaro, Dipartimento Med Sperimentale & Clin, I-88100 Catanzaro, Italy

来源：

NANO LETTERS | 2010年 / 10卷 / 03期

关键词：

Viral nanoparticle; chemical ligation; cell imaging; tumor targeting; VEGFR-1; COWPEA MOSAIC-VIRUS; EXPRESSION; PROTEIN; GROWTH; DELIVERY; SURFACE; VEGF;

D O I：

10.1021/nl1002526

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Multivalent nanoparticle platforms are attractive for biomedical applications because of their improved target specificity, sensitivity, and solubility. However, their controlled assembly remains a considerable challenge, An efficient hydrazone ligation chemistry was applied to the assembly of Cowpea mosaic virus (CPMV) nanoparticles with individually tunable levels of a VEGFR-I ligand and a fluorescent PEGylated peptide. The nanoparticles recognized VEGFR-I on endothelial cell lines and VEGFR I-expressing tumor xenografts in mice, validating targeted CPMV as a nanoparticle platform in vivo.

引用

页码：1093 / 1097

页数：5

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