An electrochemical biosensor for formaldehyde

被引:133
作者
Herschkovitz, Y [1 ]
Eshkenazi, I [1 ]
Campbell, CE [1 ]
Rishpon, J [1 ]
机构
[1] Tel Aviv Univ, Dept Mol Microbiol & Biotechnol, IL-69978 Tel Aviv, Israel
关键词
formaldehyde; biosensors; flow-cell; formaldehyde dehydrogenase; Os(bpy)(2)-poly(vinylpyridine);
D O I
10.1016/S0022-0728(00)00170-4
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
This paper reports the development of a novel detection method, based on the coupling of a biosensor measuring device and a flow-injection system, using the enzyme formaldehyde dehydrogenase and a Os(bpy)(2)-poly(vinylpyridine) (POs-EA) chemically modified screen-printed electrode. The sensor can detect 30 ng ml(-1) of formaldehyde in aqueous solution (corresponding to sub-ppb atmospheric concentrations of formaldehyde). The sensor is selective, inexpensive, stable over several days, and disposable, as well as simple to manufacture and operate. The system described here can easily be adapted to other substrates using their corresponding dehydrogenases. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:182 / 187
页数:6
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