A new efficient expression system for Bacillus and its application to production of recombinant phytase

被引：26

作者：

Kerovuo, J ^{[1
]}

von Weymarn, N

Povelainen, M

Auer, S

Miasnikov, A

机构：

[1] Danisco Cultor Innovat, Kantvik Res Ctr, Kantvik, Finland

[2] Helsinki Univ Technol, Lab Bioproc Engn, FIN-02150 Espoo, Finland

来源：

BIOTECHNOLOGY LETTERS | 2000年 / 22卷 / 16期

关键词：

Bacillus subtilis; fed-batch cultivation; phosphate-starvation; phytase; plasmid;

D O I：

10.1023/A:1005694731039

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A new expression system was developed for Bacillus subtilis. This system uses a shuttle vector (B. subtilis - Eschericia coli) carrying a phosphate starvation-inducible promoter (pst) and on a fed-batch cultivation strategy. The pst-promoter proved to be very strong and retain its tight regulation also when present on a multi-copy plasmid. The expression system developed showed promising results when applied to the production of recombinant Bacillus phytase - phytase activity at the end of cultivation reached 28.7 U ml(-1).

引用

页码：1311 / 1317

页数：7

共 19 条

[1] A PHO REGULON PROMOTER INDUCED UNDER SPORULATION CONDITIONS [J].

BIRKEY, SM ;

SUN, GF ;

PIGGOT, PJ ;

HULETT, FM .

GENE, 1994, 147 (01) :95-100

[2]

Bron S., 1990, Molecular biological methods for Bacillus, P75

[3] SEPARATE PROMOTERS DIRECT EXPRESSION OF PHOAIII, A MEMBER OF THE BACILLUS-SUBTILIS ALKALINE-PHOSPHATASE MULTIGENE FAMILY, DURING PHOSPHATE STARVATION AND SPORULATION [J].

CHESNUT, RS ;

BOOKSTEIN, C ;

HULETT, FM .

MOLECULAR MICROBIOLOGY, 1991, 5 (09) :2181-2190

[4] Phosphate-starvation-inducible proteins in Bacillus subtilis: A two-dimensional gel electrophoresis study [J].

Eymann, C ;

Mach, H ;

Harwood, CR ;

Hecker, M .

MICROBIOLOGY-UK, 1996, 142 :3163-3170

[5]

Hinnen A, 1989, Biotechnology, V13, P193

[6]

HULETT FM, 1994, PHOSPHATE IN MICROORGANISMS, P50

[7]

HULETT FM, 1995, 2 COMPONENT SIGNAL T, P289

[8]

Kerovuo J, 1998, APPL ENVIRON MICROB, V64, P2079

[9] Enzymatic evaluation of Bacillus amyloliquefaciens phytase as a feed additive [J].

Kim, DH ;

Oh, BC ;

Choi, WC ;

Lee, JK ;

Oh, TK .

BIOTECHNOLOGY LETTERS, 1999, 21 (11) :925-927

[10] High-level expression of a recombinant thermostable phytase in Bacillus subtilis [J].

Kim, YO ;

Lee, JK ;

Oh, BC ;

Oh, TK .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1999, 63 (12) :2205-2207

← 1 2 →