Optimizing PCR positivity criterion for detection of herpes simplex virus DNA on skin and mucosa

被引：67

作者：

Magaret, Amalia S.

Wald, Anna

Huang, Meei-Li

Selke, Stacy

Corey, Larry

机构：

[1] Univ Washington, Virol Res Clin, Dept Lab Med, Seattle, WA 98122 USA

[2] Univ Washington, Dept Epidemiol, Seattle, WA 98122 USA

[3] Univ Washington, Dept Med, Seattle, WA 98122 USA

[4] Fred Hutchinson Canc Res Ctr, Program Biostat, Seattle, WA 98104 USA

[5] Fred Hutchinson Canc Res Ctr, Program Infect Dis, Seattle, WA 98104 USA

[6] Childrens Hosp & Reg Med Ctr, Seattle, WA USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2007年 / 45卷 / 05期

关键词：

D O I：

10.1128/JCM.01405-06

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In 1997, we developed a PCR assay for the detection of herpes simplex virus (HSV) DNA. Recently, we determined an optimal positivity criterion based on research specimens and a dilution study. We found that a cutoff of 50 HSV DNA copies/ml of swab specimen, a level 10-fold lower than our previous cutoff, minimizes misclassification.

引用

收藏

页码：1618 / 1620

页数：3

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