The affymetrix GeneChip® platform:: An overview

被引：109

作者：

Dalma-Weiszhausz, Dennise D. ^{[1
]}

Warrington, Janet ^{[1
]}

Tanimoto, Eugene Y. ^{[1
]}

Miyada, Garrett ^{[1
]}

机构：

[1] AFFY METRIX INC, Express Prod Dev, Santa Clara, CA USA

来源：

DNA MICROARRAYS PART A: ARRAY PLATFORMS AND WET-BENCH PROTOCOLS | 2006年 / 410卷

关键词：

D O I：

10.1016/S0076-6879(06)10001-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The intent of this chapter is to provide the reader with a review of GeneChip technology and the complete system it represents, including its versatility, components, and the exciting applications that are enabled by this platform. The following aspects of the technology are reviewed: array design and manufacturing, target preparation, instrumentation, data analysis, and both current and future applications. There are key differentiators between Affymetrix' GeneChip technology and other microarray-based methods. The most distinguishing feature of GeneChip microarrays is that their manufacture is directed by photochemical synthesis. Because of this manufacturing technology, more than a million different probes can be synthesized on an array roughly the size of a thumbnail. These numbers allow the inclusion of multiple probes to interrogate the same target sequence, providing statistical rigor to data interpretation. Over the years the GeneChip platform has proven to be a reliable and robust system, enabling many new discoveries and breakthroughs to be made by the scientific community.

引用

页码：3 / 28

页数：26

共 45 条

[1]

[Anonymous], GUIDE PROBE LOGARITH

[2] Profiling early infection responses:: Pseudomonas aeruginosa eludes host defenses by suppressing antimicrobial peptide gene expression [J].

Apidianakis, Y ;

Mindrinos, MN ;

Xiao, WZ ;

Lau, GW ;

Baldini, RL ;

Davis, RW ;

Rahme, LG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (07) :2573-2578

[3] MLL translocations specify a distinct gene expression profile that distinguishes a unique leukemia [J].

Armstrong, SA ;

Staunton, JE ;

Silverman, LB ;

Pieters, R ;

de Boer, ML ;

Minden, MD ;

Sallan, SE ;

Lander, ES ;

Golub, TR ;

Korsmeyer, SJ .

NATURE GENETICS, 2002, 30 (01) :41-47

[4] Microarray analysis of gene expression profiles of cardiac myocytes and fibroblasts after mechanical stress, ionising or ultraviolet radiation [J].

Boerma, M ;

van der Wees, CGC ;

Vrieling, H ;

Svensson, JP ;

Wondergem, J ;

van der Laarse, A ;

Mullenders, LHF ;

van Zeeland, AA .

BMC GENOMICS, 2005, 6 (1)

[5] Unbiased mapping of transcription factor binding sites along human chromosomes 21 and 22 points to widespread regulation of noncoding RNAs [J].

Cawley, S ;

Bekiranov, S ;

Ng, HH ;

Kapranov, P ;

Sekinger, EA ;

Kampa, D ;

Piccolboni, A ;

Sementchenko, V ;

Cheng, J ;

Williams, AJ ;

Wheeler, R ;

Wong, B ;

Drenkow, J ;

Yamanaka, M ;

Patel, S ;

Brubaker, S ;

Tammana, H ;

Helt, G ;

Struhl, K ;

Gingeras, TR .

CELL, 2004, 116 (04) :499-509

[6] GeneChip analyses of global transcriptional responses of murine macrophages to the lethal toxin of Bacillus anthracis [J].

Comer, JE ;

Galindo, CL ;

Chopra, AK ;

Peterson, JW .

INFECTION AND IMMUNITY, 2005, 73 (03) :1879-1885

[7] High-throughput variation detection and genotyping using microarrays [J].

Cutler, DJ ;

Zwick, ME ;

Carrasquillo, MM ;

Yohn, CT ;

Tobin, KP ;

Kashuk, C ;

Mathews, DJ ;

Shah, NA ;

Eichler, EE ;

Warrington, JA ;

Chakravarti, A .

GENOME RESEARCH, 2001, 11 (11) :1913-1925

[8] Cytosolic ascorbate peroxidase 1 is a central component of the reactive oxygen gene network of Arabidopsis [J].

Davletova, S ;

Rizhsky, L ;

Liang, HJ ;

Zhong, SQ ;

Oliver, DJ ;

Coutu, J ;

Shulaev, V ;

Schlauch, K ;

Mittler, R .

PLANT CELL, 2005, 17 (01) :268-281

[9] Dynamic model based algorithms for screening and genotyping over 100K SNPs on oligonucleotide microarrays [J].

Di, XJ ;

Matsuzaki, H ;

Webster, TA ;

Hubbell, E ;

Liu, GY ;

Dong, SL ;

Bartell, D ;

Huang, J ;

Chiles, R ;

Yang, G ;

Shen, MM ;

Kulp, D ;

Kennedy, GC ;

Mei, R ;

Jones, KW ;

Cawley, S .

BIOINFORMATICS, 2005, 21 (09) :1958-1963

[10] Translation of a small subset of Caenorhabditis elegans mRNAs is dependent on a specific eukaryotic translation initiation factor 4E isoform [J].

Dinkova, TD ;

Keiper, BD ;

Korneeva, NL ;

Aamodt, EJ ;

Rhoads, RE .

MOLECULAR AND CELLULAR BIOLOGY, 2005, 25 (01) :100-113

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