The affymetrix GeneChip® platform:: An overview

被引：109

作者：

Dalma-Weiszhausz, Dennise D. ^{[1
]}

Warrington, Janet ^{[1
]}

Tanimoto, Eugene Y. ^{[1
]}

Miyada, Garrett ^{[1
]}

机构：

[1] AFFY METRIX INC, Express Prod Dev, Santa Clara, CA USA

来源：

DNA MICROARRAYS PART A: ARRAY PLATFORMS AND WET-BENCH PROTOCOLS | 2006年 / 410卷

关键词：

D O I：

10.1016/S0076-6879(06)10001-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The intent of this chapter is to provide the reader with a review of GeneChip technology and the complete system it represents, including its versatility, components, and the exciting applications that are enabled by this platform. The following aspects of the technology are reviewed: array design and manufacturing, target preparation, instrumentation, data analysis, and both current and future applications. There are key differentiators between Affymetrix' GeneChip technology and other microarray-based methods. The most distinguishing feature of GeneChip microarrays is that their manufacture is directed by photochemical synthesis. Because of this manufacturing technology, more than a million different probes can be synthesized on an array roughly the size of a thumbnail. These numbers allow the inclusion of multiple probes to interrogate the same target sequence, providing statistical rigor to data interpretation. Over the years the GeneChip platform has proven to be a reliable and robust system, enabling many new discoveries and breakthroughs to be made by the scientific community.

引用

页码：3 / 28

页数：26

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