Alteration of sterols and steryl esters in vegetable oils during industrial refining

被引：28

作者：

Ferrari, RA

Esteves, W

Mukherjee, KD

Schulte, E

机构：

[1] BAGKF, HP Kaufmann Inst, Inst Biochem & Technol Lipids, D-48147 Munster, Germany

[2] Univ Campinas, Lab Oils & Fats, BR-13081 Campinas, Brazil

[3] Univ Munster, Inst Food Chem, D-48149 Munster, Germany

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1997年 / 45卷 / 12期

关键词：

industrial fat refining; HPLC; sterols; steryl esters; vegetable oils;

D O I：

10.1021/jf970525j

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Steryl esters of fatty acids have been isolated from vegetable oils by rapid chromatography on a short column of silica gel and quantitatively determined by HPLC on RP-18 columns using an evaporative light scattering detector that was calibrated with beta-sitosteryl oleate as external standard. The steryl esters isolated according to the above technique from corn, soybean, and rapeseed oils at different stages of industrial refining have been analyzed for their constituent sterols and fatty acids by GC. Moreover, the total sterols were analyzed by GC. In all three oils a substantial part of the sterols occurred as steryl esters. Some alterations in the composition of the constituent sterols and fatty acids of the steryl esters were observed during industrial processing.

引用

页码：4753 / 4757

页数：5

共 16 条

[1] STEROLS (ISOLATION AND GC-DETERMINATION) - SURVEY BY A WORKING PARTY OF THE DGF, 114TH REPORT - GERMAN STANDARD METHODS FOR INVESTIGATION OF FATS, FATTY PRODUCTS, TENSIDES AND RELATED MATERIALS, 86TH REPORT - ANALYSIS OF MINOR COMPONENTS OF LIPIDS .7. [J].