Development of a real-time RT-PCR assay for a novel influenza A (H1N1) virus

被引：22

作者：

Jiang, Tao ^{[1
]}

Kang, XiaoPing ^{[1
]}

Deng, Yongqiang ^{[1
]}

Zhao, Hui ^{[1
]}

Li, Xiaofeng ^{[1
]}

Yu, Xuedong ^{[1
]}

Yu, Man ^{[1
]}

Qin, Ede ^{[1
]}

Zhu, Qingyu ^{[1
]}

Yang, Yinhui ^{[1
]}

Qin, Chengfeng ^{[1
]}

机构：

[1] Beijing Inst Microbiol & Epidemiol, State Key Lab Pathogen & Biosecur, Beijing 100071, Peoples R China

来源：

JOURNAL OF VIROLOGICAL METHODS | 2010年 / 163卷 / 02期

关键词：

Pandemic; Influenza A virus; H1N1; Real-time RT-PCR; TRANSMISSION; PATHOGENESIS;

D O I：

10.1016/j.jviromet.2009.09.021

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A pandemic caused by a novel influenza A virus (H1N1) poses a serious public health threat. In this study, a real-time reverse transcriptase PCR (RT-PCR) assay based on the hemagglutinin gene was developed that discriminates the novel H1N1 from swine influenza virus, seasonal H1N1/H3N2 virus and the highly pathogenic H5N1 avian influenza virus. The sensitivity of this assay was 0.2 50% tissue culture infective dose of virus and 200 copies of in vitro-transcribed target RNA. Three hundred and forty-eight clinical specimens from suspected H1N1 patients were tested using this assay, and forty-two (12.07%) were found to be positive. Tests using the real-time PCR assay recommended by WHO and virus isolation gave identical results. This sensitive and specific real-time RT-PCR assay will contribute to the early diagnosis and control of the emerging H1N1 influenza pandemic. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：470 / 473

页数：4

共 16 条

[1]

[Anonymous], PAND H1N1 2009 UPD 5

[2]

Centers for Disease Control and Prevention (CDC), 2009, MMWR Morb Mortal Wkly Rep, V58, P521

[3] Trial of 2009 Influenza A (H1N1) Monovalent MF59-Adjuvanted Vaccine. [J].

Clark, Tristan W. ;

Pareek, Manish ;

Hoschler, Katja ;

Dillon, Helen ;

Nicholson, Karl G. ;

Groth, Nicola ;

Stephenson, Iain .

NEW ENGLAND JOURNAL OF MEDICINE, 2009, 361 (25) :2424-2435

[4]

Ellis J, 2009, EUROSURVEILLANCE, V14

[5] Response to a Monovalent 2009 Influenza A (H1N1) Vaccine. [J].

Greenberg, Michael E. ;

Lai, Michael H. ;

Hartel, Gunter F. ;

Wichems, Christine H. ;

Gittleson, Charmaine ;

Bennet, Jillian ;

Dawson, Gail ;

Hu, Wilson ;

Leggio, Connie ;

Washington, Diane ;

Basser, Russell L. .

NEW ENGLAND JOURNAL OF MEDICINE, 2009, 361 (25) :2405-2413

[6] Cross-Reactive Antibody Responses to the 2009 Pandemic H1N1 Influenza Virus. [J].

Hancock, Kathy ;

Veguilla, Vic ;

Lu, Xiuhua ;

Zhong, Weimin ;

Butler, Ebonee N. ;

Sun, Hong ;

Liu, Feng ;

Dong, Libo ;

DeVos, Joshua R. ;

Gargiullo, Paul M. ;

Brammer, T. Lynnette ;

Cox, Nancy J. ;

Tumpey, Terrence M. ;

Katz, Jacqueline M. .

NEW ENGLAND JOURNAL OF MEDICINE, 2009, 361 (20) :1945-1952

[7] In vitro and in vivo characterization of new swine-origin H1N1 influenza viruses [J].

Itoh, Yasushi ;

Shinya, Kyoko ;

Kiso, Maki ;

Watanabe, Tokiko ;

Sakoda, Yoshihiro ;

Hatta, Masato ;

Muramoto, Yukiko ;

Tamura, Daisuke ;

Sakai-Tagawa, Yuko ;

Noda, Takeshi ;

Sakabe, Saori ;

Imai, Masaki ;

Hatta, Yasuko ;

Watanabe, Shinji ;

Li, Chengjun ;

Yamada, Shinya ;

Fujii, Ken ;

Murakami, Shin ;

Imai, Hirotaka ;

Kakugawa, Satoshi ;

Ito, Mutsumi ;

Takano, Ryo ;

Iwatsuki-Horimoto, Kiyoko ;

Shimojima, Masayuki ;

Horimoto, Taisuke ;

Goto, Hideo ;

Takahashi, Kei ;

Makino, Akiko ;

Ishigaki, Hirohito ;

Nakayama, Misako ;

Okamatsu, Masatoshi ;

Takahashi, Kazuo ;

Warshauer, David ;

Shult, Peter A. ;

Saito, Reiko ;

Suzuki, Hiroshi ;

Furuta, Yousuke ;

Yamashita, Makoto ;

Mitamura, Keiko ;

Nakano, Kunio ;

Nakamura, Morio ;

Brockman-Schneider, Rebecca ;

Mitamura, Hiroshi ;

Yamazaki, Masahiko ;

Sugaya, Norio ;

Suresh, M. ;

Ozawa, Makoto ;

Neumann, Gabriele ;

Gern, James ;

Kida, Hiroshi .

NATURE, 2009, 460 (7258) :1021-U110

[8] Transmission and Pathogenesis of Swine-Origin 2009 A(H1N1) Influenza Viruses in Ferrets and Mice [J].

Maines, Taronna R. ;

Jayaraman, Akila ;

Belser, Jessica A. ;

Wadford, Debra A. ;

Pappas, Claudia ;

Zeng, Hui ;

Gustin, Kortney M. ;

Pearce, Melissa B. ;

Viswanathan, Karthik ;

Shriver, Zachary H. ;

Raman, Rahul ;

Cox, Nancy J. ;

Sasisekharan, Ram ;

Katz, Jacqueline M. ;

Tumpey, Terrence M. .

SCIENCE, 2009, 325 (5939) :484-487

[9] Pathogenesis and Transmission of Swine-Origin 2009 A(H1N1) Influenza Virus in Ferrets [J].

Munster, Vincent J. ;

de Wit, Emmie ;

van den Brand, Judith M. A. ;

Herfst, Sander ;

Schrauwen, Eefje J. A. ;

Bestebroer, Theo M. ;

van de Vijver, David ;

Boucher, Charles A. ;

Koopmans, Marion ;

Rimmelzwaan, Guus F. ;

Kuiken, Thijs ;

Osterhaus, Albert D. M. E. ;

Fouchier, Ron A. M. .

SCIENCE, 2009, 325 (5939) :481-483

[10] Emergence and pandemic potential of swine-origin H1N1 influenza virus [J].

Neumann, Gabriele ;

Noda, Takeshi ;

Kawaoka, Yoshihiro .

NATURE, 2009, 459 (7249) :931-939

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