Identification of HsORC4, a member of the human origin of replication recognition complex

被引：66

作者：

Quintana, DG ^{[1
]}

Hou, ZH ^{[1
]}

Thome, KC ^{[1
]}

Hendricks, M ^{[1
]}

Saha, P ^{[1
]}

Dutta, A ^{[1
]}

机构：

[1] HARVARD UNIV,BRIGHAM & WOMENS HOSP,SCH MED,DEPT PATHOL,DIV MOL ONCOL,BOSTON,MA 02115

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 1997年 / 272卷 / 45期

关键词：

D O I：

10.1074/jbc.272.45.28247

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new member of human origin recognition complex (ORC) has been cloned and identified as the human homologue of Saccharomyces cerevisiae ORC4. HsORC4 is a 45-kDa protein encoded by a 2.2-kilobase mRNA whose amino acid sequence is 29% identical to ScORC4. HsORC4 has a putative nucleotide triphosphate binding motif that is not seen in ScORC4. HsORC4P also reveals an unsuspected homology to the ORC1-Cdc18 family of proteins, HsORC4 mRNA expression and protein levels remain constant through the cell cycle. HsORC4P is coimmunoprecipitated from cell extracts with another subunit of human ORC, HsORC2P, consistent with it being a part of the putative human origin recognition complex.

引用

页码：28247 / 28251

页数：5

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