Determination of underivatised sterols and bile acid trimethyl silyl ether methyl esters by gas chromatography-mass spectrometry-single ion monitoring in faeces

被引：87

作者：

Keller, S ^{[1
]}

Jahreis, G ^{[1
]}

机构：

[1] Univ Jena, Inst Nutr, D-07743 Jena, Germany

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2004年 / 813卷 / 1-2期

关键词：

sterols; bile acids;

D O I：

10.1016/j.jchromb.2004.09.046

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for quantification of total faccal sterols and bile acids (BAs) in human stool by using gas chromatography-mass spectrometry-single ion monitoring (GC-MS-SIM) is described. Cholesterol, coprostanol, coprostanone, cholestanol, iso-lithocholic acid (iso-LCA), lithocholic acid (LCA), iso-deoxycholic acid (iso-DCA), deoxycholic acid (DCA), chenodeoxycholic acid (CDCA), cholic acid (CA), and 12-oxo-deoxycholic acid (12-oxo-DCA) in faeces of 86 healthy subjects were determined. The sample preparation for sterol analysis requires hydrolysis and liquid extraction from matrix, but no derivatisation. The GC-flame ionisation detection (FID) and total ion current (TIC) in GC-MS were not sufficient for sterol and BA determination, whereas selectivity and specificity of the GC-MS-SIM ensured the analysis of sterols and BAs in faeces. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：199 / 207

页数：9

共 45 条

[1]

ARCA M, 1983, J LIPID RES, V24, P332

[2] ANALYSIS OF FECAL NEUTRAL STEROLS IN PATIENTS WITH FAMILIAL ADENOMATOUS POLYPOSIS BY GAS-CHROMATOGRAPHY MASS-SPECTROMETRY [J].