The Arabidopsis DELAYED DEHISCENCE1 gene encodes an enzyme in the jasmonic acid synthesis pathway

被引:416
作者
Sanders, PM
Lee, PY
Biesgen, C
Boone, JD
Beals, TP
Weiler, EW
Goldberg, RB [1 ]
机构
[1] Univ Calif Los Angeles, Dept Mol Cell & Dev Biol, Los Angeles, CA 90095 USA
[2] Ruhr Univ Bochum, Lehrstuhl Pflanzenphysiol, D-44780 Bochum, Germany
关键词
D O I
10.1105/tpc.12.7.1041
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
delayed dehiscence1 is an Arabidopsis T-DNA mutant in which anthers release pollen grains too late for pollination to occur. The delayed dehiscence1 defect is caused by a delay in the stomium degeneration program. The gene disrupted in delayed dehiscence1 encodes 12-oxophytodienoate reductase, an enzyme in the jasmonic acid biosynthesis pathway. We rescued the mutant phenotype by exogenous application of jasmonic acid and obtained seed set from previously male-sterile plants. In situ hybridization studies showed that during the early stages of floral development, DELAYED DEHISCENCE1 mRNA accumulated within all floral organs. Later, DELAYED DEHISCENCE1 mRNA accumulated specifically within the pistil, petals, and stamen filaments. DELAYED DEHISCENCE1 mRNA was not detected in the stomium and septum cells of the anther that are involved in pollen release. The T-DNA insertion in delayed dehiscence1 eliminated both DELAYED DEHISCENCE1 mRNA accumulation and 12-oxophytodienoate reductase activity. These experiments suggest that jasmonic acid signaling plays a role in controlling the time of anther dehiscence within the flower.
引用
收藏
页码:1041 / 1061
页数:21
相关论文
共 52 条
  • [1] Recent advances in brassinosteroid molecular genetics
    Altmann, T
    [J]. CURRENT OPINION IN PLANT BIOLOGY, 1998, 1 (05) : 378 - 383
  • [2] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
    Altschul, SF
    Madden, TL
    Schaffer, AA
    Zhang, JH
    Zhang, Z
    Miller, W
    Lipman, DJ
    [J]. NUCLEIC ACIDS RESEARCH, 1997, 25 (17) : 3389 - 3402
  • [3] ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999
  • [4] An Arabidopsis brassinosteroid-dependent mutant is blocked in cell elongation
    Azpiroz, R
    Wu, YW
    LoCascio, JC
    Feldmann, KA
    [J]. PLANT CELL, 1998, 10 (02) : 219 - 230
  • [5] A novel cell ablation strategy blocks tobacco anther dehiscence
    Beals, TP
    Goldberg, RB
    [J]. PLANT CELL, 1997, 9 (09) : 1527 - 1545
  • [6] A Plasmid Rescue Technique for the Recovery of Plant DNA Disrupted by T-DNA Insertion
    Behringer, Friedrich J.
    Medford, June I.
    [J]. PLANT MOLECULAR BIOLOGY REPORTER, 1992, 10 (02) : 190 - 198
  • [7] Two methyl jasmonate-insensitive mutants show altered expression of AtVsp in response to methyl jasmonate and wounding
    Berger, S
    Bell, E
    Mullet, JE
    [J]. PLANT PHYSIOLOGY, 1996, 111 (02) : 525 - 531
  • [8] Structure and regulation of OPR1 and OPR2, two closely related genes encoding 12-oxophytodienoic acid-10,11-reductases from Arabidopsis thaliana
    Biesgen, C
    Weiler, EW
    [J]. PLANTA, 1999, 208 (02) : 155 - 165
  • [9] ANTHER DEHISCENCE IN LYCOPERSICON-ESCULENTUM MILL
    BONNER, LJ
    DICKINSON, HG
    [J]. NEW PHYTOLOGIST, 1989, 113 (01) : 97 - 115
  • [10] The Arabidopsis dwarf1 mutant is defective in the conversion of 24-methylenecholesterol to campesterol in brassinosteroid biosynthesis
    Choe, S
    Dilkes, BP
    Gregory, BD
    Ross, AS
    Yuan, H
    Noguchi, T
    Fujioka, S
    Takatsuto, S
    Tanaka, A
    Yoshida, S
    Tax, FE
    Feldmann, KA
    [J]. PLANT PHYSIOLOGY, 1999, 119 (03) : 897 - 907