Determination of thiols following their separation by CZE with amperometric detection at a carbon electrode

被引：65

作者：

Wang, A ^{[1
]}

Zhang, L ^{[1
]}

Zhang, S ^{[1
]}

Fang, Y ^{[1
]}

机构：

[1] E China Normal Univ, Dept Chem, Shanghai 20062, Peoples R China

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2000年 / 23卷 / 2-3期

关键词：

capillary zone electrophoresis; amperometric detection; thiols; cysteine; glutathione; methimazole; 6-thiopurine;

D O I：

10.1016/S0731-7085(00)00326-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The amperometric detection (AD) employing a carbon disk electrode as a working electrode to determine the thiol compounds, including cysteine (CYS), glutathione (GSH), 6-thiopurine (TP), and methimazole (MMI), following their separation by capillary zone electrophoresis (CZE) is described in this paper. The detection potential was chosen at +1100 mV and all analytes exhibit good response at this potential on the carbon disk electrode. The reproducibility, linearity, and recovery were evaluated under the optimum conditions. The four analytes can be baseline resolved within 20 min and the detection limits reached about 10(-6) mol/l of magnitude. The method was applied to the separation and determination of the actual thiol samples and the analytical results were satisfactory. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：429 / 436

页数：8

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