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Analysis of urinary nucleosides.: IV.: Identification of urinary purine nucleosides by liquid chromatography/electrospray mass spectrometry

被引：32
作者：
Dudley, E
Lemière, F
Van Dongen, W
Tuytten, R
El-Sharkawi, S
Brenton, AG
Esmans, EL
Newton, RP
机构：
[1] Univ Coll Swansea, Sch Biol Sci, Biochem Grp, Swansea SA2 8PP, W Glam, Wales
[2] Univ Coll Swansea, BAMS Facil, Swansea SA2 8PP, W Glam, Wales
[3] Univ Antwerp, Dept Chem, Nucleoside & Mass Spectrometry Res Unit, B-2020 Antwerp, Belgium
[4] Singleton Hosp Trust, Oncol Unit, Swansea SA2 8QA, W Glam, Wales
[5] Univ Coll Swansea, Mass Spectrometry Res Unit, Dept Chem, Swansea SA2 8PP, W Glam, Wales
来源：
RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 2004年 / 18卷 / 22期
关键词：
D O I：
10.1002/rcm.1685
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Modified urinary nucleosides are potentially invaluable in cancer diagnosis. High-performance liquid chromatography (HPLC) was combined with full scan mass spectrometry (MS), tandem mass spectrometry and MSn analysis in order to identify purine nucleosides purified from urine. UV peaks evident in the chromatogram were examined by the various mass spectrometric techniques and adenosine, 1-methyladenosine, xanthosine, N-1-methylguanosine, N-2-methylguanosine, N-2,N-2-dimethylguanosine, N-2,N-2,N-7-trimethylguanosine, inosine, and 1-methylinosine were each identified in the urine samples from cancer patients. The benefits of the use of LC/MS compared with HPLC alone are discussed. Copyright (C) 2004 John Wiley Sons, Ltd.
引用
收藏
页码：2730 / 2738
页数：9
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