Automated determination of folate catabolites in human biofluids (urine, breast milk and serum) by on-line SPE-HILIC-MS/MS

被引：23

作者：

Alvarez-Sanchez, B. ^{[1
,2
]}

Priego-Capote, F. ^{[1
,2
]}

Mata-Granados, J. M. ^{[1
,2
,3
]}

Luque de Castro, M. D. ^{[1
,2
]}

机构：

[1] Univ Cordoba, Dept Analyt Chem, E-14071 Cordoba, Spain

[2] Univ Cordoba, Reina Sofia Hosp, Inst Biomed Res Maimonides IMIBIC, E-14071 Cordoba, Spain

[3] Sanyres Grp, Sanyres I D I Dept, Cordoba, Spain

来源：

JOURNAL OF CHROMATOGRAPHY A | 2010年 / 1217卷 / 28期

关键词：

Folic acid; Catabolite; p-Aminobenzoylglutamate; Hydrophilic interaction liquid chromatography; Anion exchange solid-phase extraction; Multiple reaction monitoring; TANDEM MASS-SPECTROMETRY; MICROBIOLOGIC ASSAY; BIOLOGICAL SAMPLES; RADIOASSAY;

D O I：

10.1016/j.chroma.2010.05.010

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid, precise and fully-automated method for analysis of folate (vitamin B9) and its catabolites (viz. p-aminobenzoylglutamate and its acetamide derivative) in biofluids is here presented. The method is based on on-line hyphenation of solid-phase extraction (SPE) by a Prospekt 2 system with hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS). The method was analytically characterized by estimation of repeatability (RSD, n = 5, between 0.5 and 4.1%), accuracy (between 96 and 105%). and sensitivity (limits of quantificantion between 0.3 and 8.3 ng/mL (1.1 and 18.8 pmol/mL) or 0.03 and 0.83 ng (0.11 and 1.88 pmol)). The proposed method is suited for routine analysis of folate catabolites as biomarkers to monitor deficiency of vitamin B9. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：4688 / 4695

页数：8

共 20 条

[1] Metabolomics analysis II. Preparation of biological samples prior to detection [J].