Identification of a Drosophila Myb-E2F2/RBF transcriptional repressor complex

被引：226

作者：

Lewis, PW

Beall, EL

Fleischer, TC

Georlette, D

Link, AJ

Botchan, MR ^{[1
]}

机构：

[1] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA

[2] Vanderbilt Univ, Sch Med, Dept Microbiol & Immunol, Nashville, TN 37232 USA

来源：

GENES & DEVELOPMENT | 2004年 / 18卷 / 23期

关键词：

Drosophila; transcription; Myb; E2F; RB; synMuv;

D O I：

10.1101/gad.1255204

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The Drosophila Myb complex has roles in both activating and repressing developmentally regulated DNA replication. To further understand biochemically the functions of the Myb complex, we fractionated Drosophila embryo extracts relying upon affinity chromatography. We found that E2F2, DP, RBF1, RBF2, and the Drosophila homolog of LIN-52, a class B synthetic multivulva (synMuv) protein, copurify with the Myb complex components to form the Myb-MuvB complex. In addition, we found that the transcriptional repressor protein, lethal (3) malignant brain tumor protein, L(3)MBT, and the histone deacetylase, Rpd3, associated with the Myb-MuvB complex. Members of the Myb-MuvB complex were localized to promoters and were shown to corepress transcription of developmentally regulated genes. These and other data now link together the Myb and E2F2 complexes in higher-order assembly to specific chromosomal sites for the regulation of transcription.

引用

页码：2929 / 2940

页数：12

共 60 条

[21] G1 cyclin-dependent kinases are insufficient to reverse dE2F2-mediated repression [J].