Enhanced photosynthesis rate in genetically engineered indica rice expressing pepc gene cloned from maize

被引：109

作者：

Bandyopadhyay, A.

Datta, K.

Zhang, J.

Yang, W.

Raychaudhuri, S.

Miyao, M.

Datta, S. K.

机构：

[1] Univ Calcutta, Dept Bot, Kolkata 7000019, W Bengal, India

[2] China Agr Univ, Natl Key Lab Agrobiotechnol, Beijing, Peoples R China

[3] Natl Inst Crop Sci Rural Dev Adm RDA, Crop Physiol & Ecol Res Div, Suwon, South Korea

[4] Univ Calcutta, Dept Biophys Mol Biol & Genet, Kolkata 7000019, W Bengal, India

[5] Inst Agrobiol Sci, Tsukuba, Ibaraki, Japan

来源：

PLANT SCIENCE | 2007年 / 172卷 / 06期

关键词：

C-4; photosynthesis; phosphoenolpyruvate carboxylase gene; transgenic rice; enhanced photosynthesis;

D O I：

10.1016/j.plantsci.2007.02.016

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Phosphoenolpyruvate carboxylase (pepc) gene is known to play the key role in C-4 photosynthesis by catalyzing initial fixation Of CO2 in C4 maize. We have introduced intact maize pepc gene in indica rice by biolistic transformation. Integration of the gene was confirmed by PCR and Southern analysis. RT-PCR and Northern blot showed expression of the gene in RNA level in transgenic plants. Immunoblot studies showed the presence of 106 kDa PEPC protein in transgenic plants. The transformants showed enhancement in photosynthesis rate in high temperature conditions. (C) 2007 Elsevier Ireland Ltd. All rights reserved.

引用

页码：1204 / 1209

页数：6

共 29 条

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